Mass Spectrometry in Proteome Research

Proteomics is a developing field that has been highly enabled by the human genome project. Proteins are the products of genes, the machinery of the cells in our bodies. Protein mass spectrometry refers to the utilization of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. Two-dimensional gel electrophoresis can be used to generate cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their alterations. Proteogenomics uses mass spectrometry data to experimentally validate gene products and to assist in the process of genome annotation & comparison.

  • Electrospray ionization mass spectrometry (ESI-MS)
  • Matrix-Assisted laser desorption (MALDI-TOF-MS)
  • Liquid chromatography mass spectrometry (LC-MS)
  • Multidimensional protein identification technology
  • Protein identification and validation

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